Aminoacylase 1 (ACY1) is an intracellular soluble homodimeric zinc-binding enzyme. It is involved in the breakdown of acetylated amino acids generated during protein degradation by catalyzing the formation of free aliphatic amino acids from N-acetylated precursors. This enzyme is evolutionarily conserved between mouse and human. Mutations in the human ACY1 gene might cause inborn metabolic disorders. The amino acid sequence of mouse ACY1 is 93% and 85% identical to that of rat and human/chimpanzee.
Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions:
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well.
Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~ 10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection willincrease sensitivity by 5to 50 fold.
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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