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Aminoacylase 1 (ACY1)

Cat no: A1374-06C

Aminoacylase 1 (ACY1)

Aminoacylase (ACY1) is an intracellular soluble homodimeric zinc-binding enzyme. It is involved in the breakdown of acetylated amino acids generated during protein degradation by catalyzing the formation of free aliphatic amino acids from N-acetylated precursors. Mutations in the ACY1 gene might cause inborn metabolic disorders. The aa sequence of human ACY1 is 100%, 88% and 86% identical to that of chimpanzee, dog and mouse/rat. \n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml\nImmunoprecipitation: 25ug/ml\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMouse myeloma cells with B cells obtained from a rat immunized with purified, insect cell-derived, recombinant human aminoacylase (aa2-408).\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1374-06C

Size

100ug

Applications

ELISA, IP, WB

Hosts

Rat

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2B

Additional Info

Recognizes human Aminocyclase. Species sequence homology: chimpanzee (100%), canine (88%), mouse and rat (86%).

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