Aminoacylase 1 (ACY1) Recombinant, Human

Aminoacylase-1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and ACY1 is the first member of a new family of zinc-binding enzymes.\n\nDescription: \nRecombinant Human ACY-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing amino acids 1-408 and having a total molecular mass of 46.9kD. Recombinant Human ACY1 contains T7 tag at N-terminus and is purified by proprietary chromatographic techniques.\n\nApplications: \n

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SPECIFICATIONS

Catalog Number

A1374-05C

Size

2ug

Form

Supplied as a liquid in 10mM Tris, pH 8.0, 1mM DTT & 10% glycerol.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Mutations in ACY1, the gene encoding aminoacylase 1, cause a novel inborn error of metabolism. Am J Hum Genet 2006 Mar;78(3):401-9 2. The pig aminoacylase 1 (ACY1) and ribosomal protein L29 (RPL29)/heparin/heparan sulfate interacting protein (HIP) genes are located together at 13q21-- > q22, corresponding to human 3p21.1. Cytogenet Cell Genet 1999;86(1):56-61 3. The human chromosome 3 gene cluster ACY1-CACNA1D-ZNF64-ATP2B2 is evolutionarily conserved in Ateles paniscus chamek (Platyrrhini, Primates). Cytogenet Cell Genet 1997;77(3-4):314-7 4. A human gene encoding diazepam-binding inhibitor/acy1-CoA-binding protein: transcription and hormonal regulation in the androgen-sensitive human prostatic adenocarcinoma cell line LNCaP. DNA Cell Biol 1996 Mar;15(3):197-208 5. Structural studies of lipooligosaccharides from Haemophilus ducreyi ITM 5535, ITM 3147, and a fresh clinical isolate, ACY1: evidence for intrastrain heterogeneity with the production of mutually exclusive sialylated or elongated glycoforms. J Bacteriol 1995 Sep;177(18):5316-21 6. Synthesis and antimicrobial activity of 1-acyl-2-sulfanilylhydrazine and 1-acy1-2-(4-amino-1-naphthylsulfony1)hydrazine. Chem Pharm Bull (Tokyo) 1962 Mar;10:210-9