Amodiaquine

Amodiaquine is an antimalarial drug with effects very similar to those of chloroquine. Amodiaquine has been widely used to treat malaria. Due to reports of fatal adverse drug reactions, discontinuation or modification of its use has been suggested. Amodiaquine is a hapten of molecular weight 267.3g/mol.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000\nOptimal dilutions to be determined by the researcher.\n \nHybridoma: \nX63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1460

Size

100ul

Applications

ELISA

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.5M sodium chloride,15mM sodium azide.\n

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG2a,k

References

1. Rowell V, Rowell FJ, Baker A, Laurie D, Sidki AM (1988) A specific ELISA method for determining chloroquine in urine or dried blood spots. Bull World Health Organ 66:211-217.\n2. Shenton FC, Bots M, Menon A, Eggelte TA, de Wit M, Greenwood BM (1988) An ELISA test for detecting chloroquine in urine. Trans R Soc Trop Med Hyg 82:216-220.\n3. Escande C, Chevalier P, Verdier F, Bourdon R (1990) Sensitive radioimmunoassay and enzyme-linked immunosorbent assay for the simultaneous determination of chloroquine and its metabolites in biological fluids. J Pharm Sci 79:23-27.

Additional Info

Recognizes the non-quinoline sidechain of amodiaquine. Reacts with amodiaquine and is able to distinguish from other antimalarial drugs. Can be used for quantitation of amodiaquine in human serum or plasma with a detection limit of approximately 2ng/ml.\nCrossreactivity towards desethylchloroquine is ~1%. Crossreactivity towards desethylamodiaquine has not been tested.