AMP Activated Protein Kinase alpha, phosphorylated (Thr172) (AMPKa) (BSA and Azide Free)

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and it plays a key role in regulation of energy homeostasis. The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. AMPK also has a role in apoptosis. AMP plays an essential role in the activation of AMPK. Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172. AMPK phosphorylation by AMPKK raises AMPK activity at least 50-fold. Secondly, AMP also inhibits the dephosphorylation and inactivation of AMPK by PP2C.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000 (Incubate membrane with the diluted antibody in 5%BSA, 1XTBS, o.1% Tween-20 at 4C with gentle shaking overnight.)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1475-05C

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Dro/Arth, NHP

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1. Hardie, D. G. et al. (1998) Annu. Rev. Biochem. 67, 821-855. 2. Corton, J. M. et al. (1994) Curr. Biol. 4, 315-324. 3. Blazques, C. et al. (1989) FEBS Lett. 489, 149-153. 4. Hawley, S. A. et al. (1996) J. Biol. Chem. 271, 27879-27887. 5. Daniels, S. P. et al. (1995) FEBS Lett. 377, 421-425. 6. Minokoshi, Y. et al. (2002) Nature 415, 339-343.

Additional Info

Detects endogenous AMPK-alpha only when activated by phosphorylation at Thr172. Detects both alpha-1 and alpha-2 isoforms of the catalytic subunit. It does not detect the regulatory beta or gamma subunits. Species crossreactivity: human, mouse, rat, monkey, and drosophilia. Species sequence homology: chicken