AMP Activated Protein Kinase alpha1 (AMPK a1)

AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kD) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/ml detected AMP-activated Protein Kinase a1 in RIPA lysates from human non-stimulated A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1475-01. Proteins were visualized using an IgG (HRP) goat and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\nIncluded Positive Antigen Control:\nA0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1475-01

Size

200ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

US Biological application references: 1. Phoenix, K.N. et al., Breast Cancer Res Treat. (2009) 113;101-111. 2. Neurath, K.M. et al., (2006) Glia 53:733-743. 3. Phoenix, K. N. et al., (2012) Genes & Cancer, doi: 10.1177/1947601912452883. 1. Chen, Z-P., et al., FEBS Letts. 443: 285-289 (1999). 2. Chen, Z-P., et al., FEBS Letts. 460: 343-348 (1999).

Additional Info

Recognizes human AMPK a1, Mr 63kD. Species Crossreactivity: rat and mouse.