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AMP Activated Protein Kinase alpha1 (AMPK Subunit alpha-1, AMPK a1, AMPKa1, 5'-AMP-activated Protein Kinase Catalytic Subunit alpha-1, AMPK1, AMPK, MGC33776, MGC57364, PRKAA1)

Cat no: A1475-01D

AMP Activated Protein Kinase alpha1 (AMPK Subunit alpha-1, AMPK a1, AMPKa1, 5'-AMP-activated Protein Kinase Catalytic Subunit alpha-1, AMPK1, AMPK, MGC33776, MGC57364, PRKAA1)

AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl- CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nELISA: 0.25ug/ml\nWestern Blot: 0.5-2.5ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1475-01D

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein G afinity chromatography.

Isotype

IgG

References

1. Zhang, Q.H., et al., Genome Res. 10(10):1546-1560 (2000). 2. Stapleton, D., et al., J. Biol. Chem. 271(2):611-614 (1996).

Additional Info

Recognizes human AMPKa1. Species crssreactivity: mouse.

Alternative Names

EC=2.7.11.1, Protein Kinase AMP-activated alpha 1 Catalytic Subunit

Read more on Supplier website

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info
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