AMPA Receptor 1 Antibody and Membrane Lysate Kit\n

AMPA Receptor 1 Antibody and Membrane Lysate Kit (#LK-103) contains all controls necessary for western blot analysis.
The kit includes:
1 vial: 0.2 ml Anti-AMPA Receptor (GluR1) (extracellular) antibody (#AGC-004).\n1 vial: 40 micro g Anti-AMPA Receptor 1 (GluR1) (extracellular) control peptide antigen.
1 vial: 0.1 ml lyophilized AMPA Receptor 1 Membrane Lysate (#LX-103).
1 vial: 0.1 ml lyophilized non-injected Xenopus oocyte membrane lysate.
AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.The (A)-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.
The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being heterotetramers composed of AMPA1 with AMPA2 or AMPA2 with AMPA3, although homotetramers can also be found.
AMPA receptors are permeable to Na^{+^{, K^{+^{and Ca^{2+^{cations. The Ca^{2+^{permeability is dependent on the presence of AMPA2: presence of AMPA2 renders the channel impermeable to Ca^{2+^{. The Ca^{2+^{permeability of the AMPA2 subunit is determined by the presence of an arginine (R) at a critical site in the pore loop instead of a glutamine (Q) present in the same site in the other AMPA subunits. RNA editing determines the presence of R as oppesed to Q. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors are impermeable to Ca^{2+^{.
Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.
Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.}}}}}}}}}}}}}}

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