Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrinmediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.
Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:2,000
Immunoprecipitation: 1-2ul/400ul lysates
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
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