AMPK gamma 2 (5

AMPK plays a key role in the regulation of energy homeostasis. It acts as a metabolic stress-inducing protein kinase that switches off biosynthetic pathways when cellular ATP levels are low and when 5`-AMP rises in response to fuel limitation. AMPK is activated by high AMP and low ATP via a mechanism that involves allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase. May increase the rate of glucose oxidation in muscle by increasing the rate of glucose transport. Shows homology to yeast protein kinase-encoding gene (snf1) regulating carbohydrate metabolism. Human AMPKg2 is a 569 aa protein, which contains unique N-terminal domains and participate in binding of AMP within the AMPK complex. AMPK is a heterotrimeric complex composed of a catalytic subunit and two regulatory subunits. Three isoforms of the gamma subunit exist in the brain with gamma 2 and gamma 3 present, which is distinct from the rat liver gamma 1 isoform. The noncatalytic subunits of AMPK are related to proteins that interact with snf1p; the beta subunit is related to the SIP1/SIP2/ GAL83 family of transcription regulators and the gamma subunit to SNF4.3 Mutations in AMPKgamma 2 can cause familial hypertrophic cardiomyopathy (HCM), which provides evidence for the central role of energy compromise in disease pathogenesis.\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1475-08C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes the N-terminal region of the human AMPKg2 protein. On Western blots, it identifies a target band at ~63kD for human and ~62kD for mouse. Reactivity has been confirmed with human NGP96 neuroblastoma lysates, JEG-3 human placenta choriocarcinoma lysates, and mouse liver homogenates.