Pancreatic amylase is a digestive enzyme with a molecular weight of approximately 56kD secreted by the pancreas.
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreatic gland.
Applications:
Suitable for use in Immunoelectrophoresis and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:8000. Strong reaction to 0.15-0.30ug of antigen.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.