Amylin (DAP, Diabetes-Associated Peptide, IAP, Islet Amyloid Polypeptide, Insulinoma Amyloid Peptide, IAPP)

Amylin or islet amyloid polypeptide (IAPP) is produced in the pancreas beta cells and coreleased with insulin. The amino acid sequence shows great homology with CGRP. Amylin has been\nshown to reverse insulin inhibition of hepatic gluconeogenesis and to inhibit muscle uptake of glucose.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions: \nImmunohistochemistry (Frozen and paraffin): 1:800-1:1500 Immunohistochemistry (PAP): 1:300-1:500 using FITC with overnight incubation at 4C.\nOptimal dilutions to be dtermined by the researcher.\n\nPositive Control:\nFormalin-fixed paraffin and frozen sections of human or rat pancreas.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2275-52D

Size

50ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Rat, Fel, GP, Prc

Form

Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH20. Dilute further with 0.1M PBS, 1% BSA, 0.1% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Mulder H. et al (1997): Islet amyloid polypeptide in the gut and pancreas: Localization, ontogeny and gut motility effects. Peptides 18(6), 771-783. 2. Mulder H et al: Non-parallelism of islet amyloid polypeptide (amylin) and insulin gene expression in rat islets following dexamethasone treatment. Diabetologia 38, 395-402 (1995).

Additional Info

Recognizes Amylin. Species Crossreactivity: human, rat, feline, porcine and guinea pig.