Amylin (DAP, Diabetes-Associated Peptide, IAP, Islet Amyloid Polypeptide, Insulinoma Amyloid Peptide, IAPP)

The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides \n(CGRP1, and CGRP2) and adrenomedullin (ADM). Calcitonin is 32 aa peptide found in the parafollicular "C" cells of the thyroid in mammals. It is also found in a number of non-mammals. It regulates the mineral (calcium and phosphate) balance. Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption. CGRP is a 37-aa peptide produced by tissue specific processing of the calcitonin gene. Calcitonin is the major product in the thyroid, whereas CGRP is the major product in neural tissues. CGRP is a potent cardiovascular agent. It has structural similarity with amylin. CGRP is found in two isoforms (CGRP-I and CGRP-II) that differs only by 3 amino acids. Amylin is a 37-aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. Adrenomedullin (ADM) is a 52-aa hypotensive peptide. It has structural similarity with CGRP and amylin. ADM is produced in peripheral tissues, adrenal medulla, lung, and kidney. \n \nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 0.5-1ug/ml. Control peptide can be used to coat plates at 1ug/ml. \nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nA2275-53D: Amylin, Human\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2275-53C

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Mosselman, S., et al., FEBS lett. 247: 154 (1989). 2. Nishi, M., et al., Mol. Endocrinol. 3: 177 (1989). 3. Sanke, T., et al., J. Biol. Chem. 263(33): 17243-17246 (1988). 4. Christmanson, L., et al., FEBS lett. 267: 160 (1990). 5. Westmark, P., et al., PNAS 86: 9662 (1987). 6. McLatchie, L.M., et al., Nature 393: 333-339 (1998). 7. Nagae, T., et al., BBRC 270: 89-93 (2000). 8. Husmann, K., et al., Mol. Cell. Endocrinol. 162: 35-43 (2000).

Additional Info

Recognizes human amylin.