Amylin, Human, Control Peptide

Control peptide for A2275-53 \n\nThe calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1 and CGRP2) and adrenomedullin (ADM). Calcitonin is 32aa peptide found in the parafollicular "C" cells of the thyroid in mammals. It is also found in a number of non-mammals. It regulated the mineral (calcium and phosphate) balance. Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption. CGRP is a 37aa peptide produced by tissue specific processing of the calcitonin gene. Calcitonin is the major product in the thyroid, whereas CGRP is the major product in neural tissues. CGRP is a potent cardiovascular agent. It has structural similarity with amylin. CGRP is found in two isoforms (CGRP-I and CGRP-II) that differs only by 3 amino acids. Amylin is a 37aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. Adrenomedullin (ADM) is a 52aa hypotensive peptide. It has structural similarity with CGRP and amylin. ADM is produced in peripheral tissues, adrenal medulla, lung, and kidney. ADM has specific receptors on astrocytes and it is unregulated in ischaemia. The calcitonin family peptides probably act through G-protein coupled membrane receptors. Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1ug/ml\nAntibody Blocking: 5-10ug per 1ul A2275-53 (antiserum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A2275-53A

Size

50ug

Applications

ELISA

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide

Purity

Highly purified

References

1. Mosselman, S., et al., FEBS Lett. 247: 154 (1989). 2. Nishi, M., et al., Mol. Endocrinol. 3: 177 (1989). 3. Sanke, T., et al., JBC 263: 17,243 (1980). 4. Christmanson, L., et al., FEBS Lett. 267: 160 (1990). 5. Westmark, P., et al., PNAS 86: 9662 (1987). 6. McLatchie, L.M., et al., Nature 393: 333-339 (1998). 7. Nagae, T., et al., BBRC 270: 89-93 (2000). 8. Husmann, K., et al., Mol. Cell. Endocrinol. 162: 35-43 (2000).

Additional Info

Epitope: Full length 37aa human amylin Peptide\nSpecies Sequence Homology: Conserved in mouse, rat and various other species.