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Amyloid Beta, 1-40 (CT) (Amyloid A4)

Amyloid Beta, 1-40 (CT) (Amyloid A4)

Cat no: A2275-59E

Supplier: United States Biological
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Alzheimers disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (Ab4) protein has been found to be the principal constituent of senile plaques of ADs patients. The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4kD Ab4 protein. Two variants of Ab4 can be detected; Ab-40 short-tailed and Ab-42 long-tailed. Applications: Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using HRP/ECL detection. Recommended blocking with casein/tween 20 based buffer. Immunocytochemistry: 1-10ug/ml ELISA: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2275-59E
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder in 2X PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml dH2O.
P type: Mab
Isotype: IgG1
Purity: Purified by thiophilic adsorption and size exclusion chromatography.
Additional info: Recognizes the C-terminus of human beta Amyloid 1-40. Does not cross-react with beta Amyloid 1-42.

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