Amyloid Beta, 1-42 (CT) (Amyloid A4)

Alzheimers disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (Ab4) protein has been found to be the principal constituent of senile plaques of ADs patients. The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4kD Ab4 protein. Two variants of Ab4 can be detected; Ab-40 short-tailed and Ab-42 long-tailed.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:100-1:1000\nImmunohistochemistry (FFPE): 1:100-1:2000. Pretreat sections with 70% formic acid for 10-30 minutes. Antibody should be incubated with sections overnight at 4C.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2275-75N

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins or preservative added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Helwig M, Hoshino A, Berridge C, Lee SN, Lorenzen N, Otzen DE, Eriksen JL, and Lindberg I. The Neuroendocrine Protein 7B2 Suppresses the Aggregation of Neurodegenerative Disease-related Proteins. J. Biol. Chem.; 288: 1114 - 1124, 2013. 2. Takami M, Nagashima Y, Sano Y, Ishihara S, Morishima-Kawashima M, Funamoto S, and Ihara Y. y-Secretase: Successive Tripeptide and Tetrapeptide Release from the Transmembrane Domain of b-Carboxyl Terminal Fragment. J. Neurosci.; 29: 13042 - 13052, 2009. 3. Parvathy, S., et al., (2001),

Additional Info

Recognizes the C-terminus of human beta amyloid and is specific for the isoform ending at the 42nd amino acid. Species Crossreactivity: mouse