Amyloid, Beta (ABeta, Amyloid Beta, ABeta Precursor Protein, APP, (Biotin)

Amyloid beta (ABeta) precursor protein (APP) is a 100-140kD transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Abeta fragments form the main components of amyloid plaques in Alzheimer's disease (AD) (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137408

Size

100ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8. 2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9. 3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42. 4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22. 5. Ando, K. et al. (1999) J Neurosci 19, 4421-7. 6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.

Additional Info

Recognizes human beta-Amyloid (D2A6H). Recognizes the ABeta-37 isoform of the beta-amyloid peptides. Does not react with other beta-amyloid peptides.