Amyloid P Component, Serum, Human (SAP, APCS, PTX2, mature chain 204aa, chromosome 1q21-23)

Control for A2275-88H (antiserum) and A2275-88J (affinity purified monoclonal antibody).\n\nPentraxins family of proteins acquired the name from their ability to form pentameric (or decameric) structures formed by non-covalent interactions. C-reactive protein ( CRP or PTX1; mature chain 206aa; chromosome 1q21-23) non-glycosylated, ~24kD monomer and ~118kD pentamer) is a ubiquitous protein found in both vertebrates and invertebrates. Originally CRP was defined as a substance, observed in the plasma of patients with acute infections, that reacted with the C polysaccharide of the Pneumococcus. It is one of the plasma proteins that are called acute phase reactants because of a pronounced rise in concentration after tissue injury or inflammation. In the case of CRP, the rise may be 1000-fold or more. CRP is composed of 5 identical, 21,500MW subunits. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver. Those cells produce CRP detectable on lymphocytes. \n\nSerum amyloid P component or SAP or APCS, or PTX2 (mature chain 204aa, chromosome 1q21-23) with which CRP has about 59% homology, is situated in the same area of chromosome 1. SAP is universally present in amyloid deposits (senile plaque and neurofibrially tangels) in Alzheimers patients. SAP levels in CSF can be useful for assessing cognitive impairment in AD patients. SAP appeared not to be required for A-beta deposition since no endogenous SAP immunoreactivity was found in mice overexpressing APP. \n\nIn mice with a targeted deletion of the SAP gene, induction of reactive amyloidosis was retarded, demonstrating the participation of SAP in pathogenesis of amyloidosis in vivo and confirming that inhibition of SAP binding to amyloid fibrils is an attractive therapeutic target. SAP knock out mice develop antinuclear autoimmunity and glomerulonephritis.The exact role of SAP in SLE is not clear. SAP also neutralizes LPS. It is potentially useful in defense against serious gram-negative sepsis.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nSpecificity: \nHuman SAP purified from acute phase serum. Native form ~235kD. Monomer ~25kD. Recommend using anti-mouse SAP for detecting mouse SAP.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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