Amyloid Precursor Protein (APP, Amyloid beta (A4) Precursor Protein)

Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 aa peptides (beta amyloid1-40,1-42, and 1-43; approx. 4.5kD). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. APP gene has 19 exons (1). At least 8 different types of APP (APP695, APP751, and APP770 are the major ones) are produced as a result of alternative splicing of exons 7, 8, and 15. Exons 7 codes for a 57-aa domain that is analogous to Kunitz protease inhibitors (KPI). Exon 8 codes for a 19-aa residue domain with homology to the MRC OX-2 antigen found on the membrane of neurons, while exon 15 codes for an 18-aa domain, 16 aa proximal to the Ab region. APP695 lacks exons 7 and 8, whereas APP770 and APP751 represents the full length and the exon 8 lacking isoform. Beta amyloid A4 protein represents 597-638 aa of APP695 (28 aa from the extracellular domain and 14-15 aa from the extracellular domain). APP695 is predominantly expressed in the brain while APP751 and APP770 are also found in other tissues.\n \nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500 using ECL.\nELISA: 1:5000-1:50,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2275-69R

Size

50ul

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kang J et al (1987) Nature 325, 733; Kang J et al (1989) Nucleic Aacid Res. 17, 2130; Shivers BD et al (1988) EMBO J. 7; 1365-1370; Ponte P et al (1988) Nature 3, 31, 525-527; Schilling J et al (1991) Gene 98, 225-230; Yoshikai S et al (1987) Gene 87, 257-263; Neve R et al (1992) PNAS 89, 3448; Goldgaber D et al (1987) Science 235, 877; Yankner B et al (1989) Science 245, 417; Golde T et al (1992) Science 255, 728.

Additional Info

The human peptide sequence is found in all major APP isoforms (APP695, APP751 and APP770). It is 95% identical to porcine, mouse and rat, 90% with chicken APPs. Species crossreactivity has not been established.

Alternative Names

A4, AAA, ABETA, ABPP, AD1, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease), Amyloid beta A4 protein precursor, APPI, Cerebral vascular amyloid peptide, CTFgamma, CVAP, PN2, PN-II, PreA4, Protease nexin-II