Amyloid Precursor Protein, Kpi Domain (APP, Amyloid beta (A4) Precursor Protein)

Only the APP-751 and APP-770 isoforms contain a KPI domain, it being absent from the APP-695 isoform that is most abundant in neuronal tissue. However, there is evidence that suggests KPI contain isoforms may be important in the pathogenesis of Alzheimer disease.\n\nApplications:\nSuitable for use in Immunohistochemistry, Immunoprecipitation, and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:400. Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. 0.05M Tris pH 7.0, followed by reduction and alkylation is recommended.\nImmunoprecipitation: Neat, 5ul/ml after reduction and alkylation\nWestern Blot: 1:500-1:1000. Detects a band of approximately 115kD in NTera2/DI cell lysates. \nOptimal working dilutions to be determined by researcher.\n\nRecommended Secondary Reagents: Sheep anti Rabbit IgG (Aff. Pur.)-HRP\n\nRecommended Positive Control Tissue: Alzheimers disease brain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2275-71P

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid containing 0.02% sodium azide.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

1. Campbell, E. et al. (1999). Methods to uncover an antibody epitope in the KPI domain of Alzheimer's amyloid precursor protein for immunohistochemistry in human brain. J. Neurosci. Meths. 93: 133-138.

Additional Info

Recognizes an epitope in the Kunitz protease inhibitor (KPI) domain of human amyloid precursor protein (APP). Species Crossreactivity: monkey