ANAPC10, NT (Anaphase-promoting Complex Subunit 10, APC10, Cyclosome Subunit 10 APC10)

Cat no: A2298-72Y1

Supplier: United States Biological

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APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	A2298-72Y1
Reactivities:	Mouse
Hosts:	Rabbit
Applications:	Immunohistochemistry, Western Blot
Size:	50ul
Form:	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
Additional info:	Recognizes mouse ANAPC10. Species sequence homology: human

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ANAPC10, NT (Anaphase-promoting Complex Subunit 10, APC10, Cyclosome Subunit 10 APC10)

Cat no: A2298-72Y1

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