Anaplastic Lymphoma Kinase (Nucleophosmin, p80, ALK, NPM, B23, NPM-ALK)

The ALK gene is carried by chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as nucleophosmin or B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre-ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM-ALK) resulting from t(2;5) translocation is a protein of 80kD with the N-terminal portion of NPM linked to the complete intracellular portion of ALK. ALK is found in many large cell lymphomas. Karpas 299 and SU-DHL-1 lymphoid cell lines strongly express NPM-ALK. \n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml \nImmunohistochemistry (Paraf?n or frozen): 5-10ug/ml. \nWestern Blot: 1-5ug/ml\nOptimal dilution to be determined by the researcher.\n\nPositive Controls:\nHuman T-cell lines harboring the NPM-ALK translocation, including Karpas 299, DHL and TS cells. \n\nNegative Controls:\nJurkat and the B-cell lines BL60, IARC, Ramos and Raji.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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