Androgen Receptor (AR)

The androgen receptor (AR) has been localized e.g. in the testis, (Sertoli and Leydig cells), prostate, foreskin epidermis, fibroblasts, breast epithelium, sweat and apocrine glands and hepatocytes. Prostate cancers are very heterogeneous with regard to AR expression, the loss of androgen responsiveness is not paralleled by the loss of AR. Studies on AR distribution might be useful to study the role of AR in related disease processes. Antigen localization is nuclear.\n\nApplications:\nSuitable for use in Immunoblot, Immunocytochemistry and Immunohistochemistry (frozen sections and paraffin-embedded tissue after mivrowave pretreatment).\n\nRecommended Dilution: \nImmunohistochemistry: 1:5-1:10; Dilute immediately before use with PBS.\n\nPositive Control: \nTestis\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2281-02C

Size

1ml

Applications

ICC, IHC, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified IgG

Isotype

IgG1

References

Jansen PJ et al. Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded tissue after microwave pretreatment. J Histochem Cytochem 42,1169-1175 (1994) Ruizeveld de Winter JA et al. Androgen receptor expression in human tissues: an immunohistochemical study. J Histochem Cytochem 39, 927-936 (1991) Zegers ND et al. Epitopic prediction and confirmation for the human androgen receptor. Generation of monoclonal antibodies for multi-assay performance following the synthetic peptide strategy. Biochem Biophys Acta 1073, 23-32 (1991)

Additional Info

Reactive with a 110kD protein in a lysate from the LNCP cell line.