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Androgen Receptor (BSA & Azide Free)

Cat no: A2281-01X

Androgen Receptor (BSA & Azide Free)

The expression of androgen receptor (AR) is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormoneresponsiveness. \n\nApplications:\nSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nImmunoprecipitation (Denatured verified): 10ug/mg protein lysate. (Use Protein A).\nWestern Blot: 5ug/ml for 2hrs at RT \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nLnCap cells \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 24 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2281-01X

Size

500ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See A2281-01

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Prins G, et al. (1991) Endocrinology 129: 3187- 3199

Additional Info

Recognizes rat Androgen Receptor. Species Crossreactivity: human and mouse.

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