Androgen Receptor, phosphorylated (Ser216)

Androgen receptor (AR) belongs to the super-family of nuclear hormone receptors that employ complex molecular mechanisms to guide the development and physiological functions of their target tissues. Extensive research over the past several years has explored the possible mechanisms of steroid receptor action. The group of co-regulators includes co-activators, co-repressors, cointegrators and mediator protein complexes. Each of these proteins potentially interacts with multiple signaling systems, including most steroid receptors. In mammals, exposure to androgens early in development is essential for masculinization of the male reproductive phenotype. Androgen stimulation results in cell proliferation in both the development of prostate and malignant prostate. Data from animal and human studies indicate that sex steroids have important skeletal effects. Androgens clearly enhance bone size, likely through effects on periosteal bone formation. Similarly to other steroid receptors, AR is a phosphoprotein, but the functional significance of the covalent receptor modification by phosphorylation has remained enigmatic.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1.0ug/ml\nWestern Blot: 0. 5-2.0ug/ml\nImmunoprecipitation: 3.0-5.0ug/extract from 106 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2281-14

Size

25ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Janne, OA. et al. Biochemical Society Transactions. 24, 4 (2000). 2. Khosla, S. et al. Endocrinol Metab Clin North Am. 32(1):195(2003). 3. Hotchkiss, AK. et al. Environ Health Perspect. 110 3, 435(2002). 4. Taneja, SS. et al. J Biol Chem. Jan 7 (in press) (2004).

Additional Info

Recognizes human AR when phosphorylated at Ser216.