Angiogenin (ANG, Ribonuclease RNase A Family 5, RNASE5)

Angiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence identity with pancreatic RNase. Like other members of the ribonuclease superfamily, angiogenin is a cytotoxic agent that can eliminate cellular protein synthesis. Angiogenin causes protein synthesis inhibition by functioning as a cytotoxic tRNA-specific RNase. Angiogenesis is the formation of blood vessels or capillaries from existing blood vessels due to specific signals. Angiogenin induces the growth of new blood vessels. It stimulates capillary and umbilical vein endothelial cells to produce diacylglycerol and secrete prostacyclin by phospholipase activation. Angiogenin exhibits both angiogenic and non-angiogenic activities and is involved in endothelial cell migration, proliferation, and differentiation. It is produced by a variety of tumor and normal cell types. Cells that express angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, normal colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. Angiogenin is also present in normal human plasma. \n\nApplication(s): \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1-2ug/ml\nWestern Blot: 1-2ug/ml, detects a band of approximately 14.4kD.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2292-45

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1\n

References

Bicknell R & Vallee BL Angiogenin stimulates endothelial cell prostacyclin secretion by activation of phospholipase A2. Proc Natl Acad Sci U S A 86:1573-7 (1989). Fett JW et al. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells. Biochemistry 24:5480-6 (1985). Kurachi K et al. Sequence of the cDNA and gene for angiogenin, a human angiogenesis factor. Biochemistry 24:5494-9 (1985).

Additional Info

Recognizes Angiogenin. Species Crosseactivity: Human. \n