Angiopoietin 1, Recombinant, Human (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)

Tie-1 (human 1138 aa; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Murine and human Ang-1 are 498aa secreted angiogenic proteins. It is only 60% homologous with Ang-2. Ang-1 is the natural ligand for Tie-2 and is primarily expressed in developing endothelial cells.\n\nSource: DNA sequence encoding human angiopoietin-1 (Ser20-Phe 498; Q5HYA0) fused with a 6X histidine tag at the C-terminus, expressed in NS0 cells.\n\nMolecular Mass: Based on N-terminal sequencing, the recombinant human angiopoietin-1 protein starts at Ser 20 and has a calculated molecular mass of 56kD. As a result of glycosylation, the recombinant protein migrates as an approximately 70kD band in SDS-PAGE under reducing conditions.\n\nPurity: (same/more than)90%, as determined by SDS-PAGE and visualized by silver stain.\n\nEndotoxin: (same/less than)1EU/ug (LAL) \n\nBiological Activity: Measured by its ability to inhibit serum deprivation induced apoptosis in human umbilical vein endothelial cells (HUVEC) (6). The ED50 for this effect is typically 10-40ng/ml in the presence of 5ug/ml of a cross-linking antibody (mouse anti-6X histidine).\n\nForm: Supplied as a lyophilized powder from 50mM Tris-citrate, 500mM sodium chloride, pH 6.5.\n\nReconstitution: Reconstitute with sterile PBS to 100ug/ml.\n\nStorage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2293-01

Size

25ug

Applications

ELISA, WB

Reactivities

Mouse

Form

Supplied as a lyophilized powder from 50mM Tris-citrate, 500mM sodium chloride, pH 6.5, 5%\ntrehalose.

Purity

>90%, as determined by SDS-PAGE and visualized by silver stain.

References

1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257. 2. Davis, S. et al. (1996) Cell 87:1161. 3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514. 4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213. 5. Teichert