Angiopoietin 1, Recombinant, Human (Ang-1)

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis. Study of tumor angiogenesis has led to the identification of several proteins in angiogenic pathway. Angiopoietin-1 is an angiogenic secreted protein that interact with endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 appears to play a crucial role in mediating matrix and mesenchyme. It mediates blood vessel maturation and stability. Ang-1 may play a critical role in heart development. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. A homolog of Ang-1, termed Angiopoietin-2 has recently been identified. It may act an antagonist for Ang-1 and Tie-2.\n\nApplications:\nSuitable for use in Western Blot only.\n\nRecommended Dilutions:\nWestern Blot: 10ul/lane (minigel). Heat the sample once prior to loading. Migrates ~70kD due to glycosylation.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2293-08F

Size

50ul

Applications

WB

Form

Supplied as a liquid in SDS-PAGE sample buffer (reduced).

Purity

Highly Purified (> 95%)

References

1. Kwak HJ et al (1999) FEBS Lett. 448, 249. 2. Davis S et al (1996) Cell 87, 1161-1169. 3. Nomura N et al (1994) DNA Res. 1, 27-35. 4. Suri, S et al (1996) Cell 87, 1171. 5. Davis, S et al (1996) cell 87, 1161. 6. Maisonpierre C et al (1997) Science 277, 55.

Additional Info

Recombinant human Ang-1 (1-498aa, Mr 55kD, Davis, et al., 1996)) was expressed as a fusion protein containing a his-tag at the C-terminus in mouse NSO cells.\nSpecies sequence homology: \nMouse and human Ang-1; 498aa: ~98%\nMouse and human Ang-2, 496aa: ~85%\nAng-1 and Ang-2: ~60%