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Angiopoietin-like 1 (ANGPTL1) (Biotin)

Cat no: A2293-41


Supplier: United States Biological
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Angiopoietin-like 1 is a secreted glycoprotein structurally related to the angiopoietins. It contains an N-terminal coiled-coiled domain and a C-terminal fibrinogen-like domain. Unlike angiopoietins which are agonists and antagonists of the Tie-2 receptor tyrosine kinase, Angiopoietin-like 1 is an orphan ligand that does not bind Tie-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/mL Detection limit is ~2ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2293-41
Reactivities: Human
Hosts: Goat
Applications: Western Blot
Conjugates: Biotin
Size: 50ug
Form: Supplied as a lyophilized powder from PBS, 50ug of BSA. Label with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography
Additional info: Recognizes human Angiopoietin-like 1. Crossreactivity: rhAngiopoietin-4; <20%, rhAngiopoietin-1 and rhAngiopoietin-2; <1%

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