Angiopoietin Like Protein 4 (ANGPTL4) BioAssay(TM) ELISA Kit (Mouse)

Angiopoietin Like Protein 4 (ANGPTL4) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANGPTL4 in mouse serum, plasma and other biological fluids.\n\nDetection Range:\n0.156-10ng/ml\n\nSensitivity:\n0.065ng/ml\n\nIntra-Assay: \nCV(same/less than)0% \n\nInter-Assay: \nCV(same/less than)12%\n\nKit Components:\n*023385A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use\n*023385B: Standard, 2x1vial\n*023385C: Detection Reagent A, 1x120ul\n*023385D: Detection Reagent B, 1x120ul\n023385E: TMB Substrate, 1x9ml\n023385F: Wash Buffer, 30X, 1x20ml\n023385G: Standard Diluent, 1x20ml\n023385H: Assay Diluent A, 1x12ml\n023385J: Assay Diluent B, 1x12ml\n023385K: Stop Solution, 1x6ml\n*023385L: Positive Control, 3.08ng/ml, 1x1vial\n\nPrecaution:\nThe Stop Solution (023385K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.\n\nNotes on 023385L - Positive Control:\n\nIntended Use:\nThe Positive Control is from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.\n\nCharacteristics:\nThe control vial contains mouse ANGPTL4 in mouse serum (lyophilized powder). Note: The mouse based serum used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).\n\nExpected Value:\n3.08ng/ml\n\nUsage: \nDissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20 degrees C. After reconstitution, store Positive Control vial at 4 degrees C and use within 5 days.\n\nStorage and Stability:\nStore *023385A, *023385B, *023385C and 023385D at -20 degrees C upon receipt. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nAssay Procedure Summary:\n1. Prepare all reagents, samples and standards.\n2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degrees C.\n3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degrees C.\n4. Aspirate and wash 3 times.\n5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C.\n6. Aspirate and wash 5 times.\n7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37 degrees C.\n8. Add 50ul Stop Solution. Read at 450nm immediately.

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