Angiostatin

Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen. Recombinant angiostatin has 260 amino acids, residues 97-357 of human proplasminogen, and a calculated molecular mass of 29.8kD.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2294-07

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute in 100ul sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sim BK, O'Reilly MS, Liang H, Fortier AH, He W, Madsen JW, Lapcevich R, Nacy CA: A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer. Cancer Res Apr;57(7):1329-34 (1997) 2. Shepard SR, Boucher R, Johnston J, Boerner R, Koch G, Madsen JW, Grella D, Sim BK, Schrimsher JL affei M., Halaas J., Ravussin E. et al.: Large-scale purification of recombinant human angiostatin. Protein Expr Purif Nov;20(2):216-27 (2000) 3. Cao Y, Veitonmaki N, Keough K, Cheng H, Lee LS, Zurakowski D: Elevated levels of urine angiostatin and plasminogen/plasmin in cancer patients. Int J Mol Med May;5(5) :547-51 (2000) 4. Ji WR, Barrientos LG, Llin

Additional Info

Recognizes human serum Angiostatin.