Angiotensin Converting Enzyme 2 (ACE-2, ACEH)

ACE-2 (also known as ACE-2 and ACE homolog, ACEH) gene has been mapped at human chromosome Xp22. ACE-2 enzymes from human (805aa) and mouse (798aa) are single chain proteins with 40% sequence homology to N- and C-terminal domains of ACE. However, in contrast to s-ACE which consists of two catalytic sites, ACE-2 contains only one active site. Unlike s-ACE and t-ACE which are dipeptidyl-carboxypeptidases, ACE-2 acts as a carboxypeptidase, cleaving single residue from Ang-I, generating Ang1-9 and a single residue from Ang-II to generate Ang1-7. ACE-2 can cleave angiotensin-I, but not bradykinin and the enzyme activity is not inhibited by the ACE inhibitors. This enzyme is expressed highly in heart, kidney and testis and moderately in colon, small intestine and ovary. ACE-2 is an essential regulator of heart fuction because targeted disruption of this enzyme in mice results in severe cardiac contractility defect, increased angiotensin-II levels and upregulation of hypoxia-induced genes in the heart.\n\nApplications: \nSuitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA (Direct and Indirect): 1:2000-1:10,000\nWestern Blot: 1:2000-1:10,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween-20 and 5% skim milk; suggested incubation time is 1 hour at RT. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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