Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that
cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The
balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal,
and pulmonary function. ACE-2 isoforms of 75kD and 120kD are differentially expressed
between lung and kidney, respectively, and a shed soluble form is generated by
TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for
the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa
sequence identity with mouse and rat ACE-2.
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 2ug/ml
Immunohistochemistry: 25ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse myeloma with B cells obtained from a mouse.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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