Angiotensin Converting Enzyme (ACE, ACE1, DCP1)

Renin-Angiotensin System (RAS) is a crtical regulator of blood pressure homeostasis. The protease renin cleaves angiotensinogen into inactive decameric peptide angiotensin-I (Ang-I). Angiotensin-converting enzyme (ACE) then cleaves C-terminal dipeptide from Ang-I to form an active octamer angiotensin-II (Ang-II), which can contribute to hypertension by promoting vascular smooth muscle vasocontriction and renal tubule sodium reabsorption. ACE can also cleave many other small peptides including the vasodialating peptide bradykinin into inactive fragment, cleave Alzheimer amyloid beta-peptide (Abeta), retard Abeta aggregation, deposition and fibril formation. ACE mutant mice display spontaneous hypotension, partial male infertility and kidney malformations. ACE is found in somatic (s-ACE) and testicular/germinal (t-ACE) isoforms. The products of renin and ACE catalysis, namely Ang1-10 and Ang1-8 can also be by another peptidase, ACE-2 to Ang1-9 and Ang1-7, respectively. ACE-2 and ACE (s-ACE and t-ACE) are made as transmembrane (TM) proteins. \n\nThese enzymes also exist as soluble, truncated forms lacking the TM and cytosolic domains. ACE (also known as dipeptidyl carboxypeptidase-1, DCP1; Kininase-II, ACE1) gene has been mapped at human chromosome 17q23. The s-ACE and t-ACE isoforms are generated by alternative splicing of ACE-2 gene. Somatic-ACE, a Zn (II) containing dipeptidyl carboxy peptidase is a single chain glycoprotein with a molecular mass of ~140kD. The s-ACE enzymes from mouse (1312aa), rat (1313aa) and human (1306aa) contain two large areas of homologous sequence, each containing catalytic site and a Zn-binding region. These homologous regions are approximately half the size of whole s-ACE. The s-ACE is expressed in many somatic tissue tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL). Recognizes >120kD somatic-ACE from native tissue.\nELISA: 0.5-1ug/ml. Coat plates at 1ug/ml with A2295-01K\nAngiotensin Converting Enzyme, Mouse, Control Peptide. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2295-01L

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Crackower, M.A., Nature 417: 822 (2002). 2.

Additional Info

Recognizes mouse Angiotensin Converting Enzyme. Species sequence homology: human-87% and rat-.81% No significant sequence homolgy exist with the t-ACE of any species.