Angiotensin Converting Enzyme (ACE, Angiotensin I Converting Enzyme (Peptidyl-dipeptidase A) 1, ACE1, CD143, Dipeptidyl Carboxypeptidase I, DCP1, DCP, Kininase II, MGC26566, MVCD3) (FITC)

CD143 is also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul labels of a neat-1:10 working dilution to 10e6 cells in 100ul. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

A2294-98C

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 0.09% sodium azide, 1.0% BSA. Labeled with FITC.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Donilov, S.M. et al. (1994) Structure-function analysis of angiotensin-converting enzyme \nusing monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806-26814. 2. Metzger, R. et al. (2000) CD143 in the development of atherosclerosis. Atherosclerosis 150:21-31.

Additional Info

Recognizes human CD143 and active ACE binding to an N-terminal domain epitope different to that recognized by clone 9B9.

Alternative Names

Angiotensin-converting Enzyme, Soluble Form, EC=3.4.15.1, EC=3.2.1.-