Angiotensin Converting Enzyme (ACE, CD143)

Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate\n\nFusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the mouse X36-Ag8-653 myeloma cell line.\n\nRecommended Negative Controls: Mouse IgG1 Negative Control\n\nApplications:\nFlow Cytometry: Neat -1:10\nImmunohistochemistry: (frozen)- Neat-1:30\nELISA\nImmunoprecipitation \nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. \nImmunohistochemistry: Recommended positive control tissue: Kidney.

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SPECIFICATIONS

Catalog Number

A2295-02A

Size

1ml

Applications

ELISA, FC, IHC, IP

Hosts

Mouse

Reactivities

Hum, NHP

Form

Tissue Culture Supernatant. Supplied as a liquid with 0.1% sodium azide.

P Type

Mab

Purity

Tissue culture supernatant.

Isotype

IgG1

References

1. Danilov, S.M. et al. (1994). Structure - funstion analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806 - 26814. 2. Metzger, R.

Additional Info

Recognizes human CD143, also known as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithehal cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. This antibody recognizes active ACE binding to an N-terminal domain epitope different to that recognized by clone 9B9. Species Crossreactivity: Human, monkey