Angiotensin Converting Enzyme (ACE, CD143)

CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. \n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50-1:100\nImmunohistochemistry (Paraffin): Neat. 1:20. Requites antigen retrieval using heat treatment methods prior to staining.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nSpleen cells from immunized Balb/c mice were fused with cells of the mouse X63-Ag8-653 myeloma cell line.\n\nRecommended Positive Control Tissue: \nKidney (lung in rat studies) \n\nRecommended Negative Control:\nI1904-79G: IgG1, Mouse, Negative Control\n\nRecommended Secondary Reagents: \nI1904-65W: IgG, F(ab

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SPECIFICATIONS

Catalog Number

A2295-02C

Size

1ml

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Fel, GP, NHP, Prc, Rabbit

Form

Supplied as a liquid with 0.1% sodium azide.

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG1

References

1. Metzger, R. et al. (1999) Angiotensin-converting enzyme in non-neoplastic kidney diseases Kidney International 56: 1442-1454. 2. Balyasnikova, I.V. et al. (2003) Monoclonal antibodies to denatured ACE (CD143) broad species specificity, reactivity on paraffin sections and detection of subtle conformational changes in the C-terminal domain of ACE. Tissue Antigens. 61 (1): 49- 62.

Additional Info

Recognizes human CD143, also know as angiotensin-converting enzyme (ACE). This clone recognizes denatured ACE, binding to an epitope in the C domain and has been reported to be active in Western Blot. Species Crossreactivity: monkey, rabbit , bovine, canine, feline, guinea pig, rat, porcine and mouse