Angiotensin Converting Enzyme (ACE, CD143) (PE)

Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the mouse X63-Ag8-653 myeloma cell line.\n\nRecommended Negative Controls: Mouse IgG1 Negative Control\n\nApplications:\nFlow Cytometry: Neat\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.

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SPECIFICATIONS

Catalog Number

A2295-02M

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum, Rat, Fel, NHP

Form

Purified IgG conjugated to R. Phycoerythr(RPE) - lyophilized with 0.1% sodium azide, 1% BSA. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Danilov, S. M. et al. (1994). Structure - Function analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269:26806

Additional Info

Recognizes human CD143, also know as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expresssed by spermatozoa. This antibody recognizes active ACE binding to an N-terminal domain epitope, different to that recognized by clone i2H5. Species Crossreactivity: Human, monkey (3), rat (3), hamster, feline