Angiotensin II Type 1 Receptor (AT1)

Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). Major cardiovascular effects appear to be mediated through AT1. AT1 has three isoforms (rat AT1A 359aa; AT1B/AT III, 359aa; and AT1C, 177aa). Rat AT1 receptors are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular. The C-terminus is inside the membrane (cytoplasmic). The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system. AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle. AT2 gene ( chromosome x) encodes 363 aa protein. It is highly expressed in myometrium with lower levels in adrenal and fallopian tube. It is also expressed at high levels in fetal kidney and intestine. Rat AT1 receptors are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular, while C-terminus inside the membrane (cytoplasmic). The binding of angiotensin II with AT1 receptors activate a phosphatidylinositol-calcium second messenger system. AT1 receptors are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle. AT2 gene (chromosome x) encodes 363aa protein. It is expressed at high levels in myometrium, fetal kidney and intestine. It is expressed at lower levels in adrenal and fallopian tube.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 (ECL). Detected ~45kD band.\nELISA: 1:10,000-1:100,000 using 50-100ng of A2295-07G Control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2295-07D

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Murphy, T.J., et al., Nature 351: 233 (1991). 2. Iwai, N., et al., BBRC 177: 299 (1991). 3. Langford, K., et al., BBRC 183: 1025 (1992). 4. Sasamura, H., et al., BBRC 185: 253 (1992). 5. Yoshida, H., et al., BBRC 186: 1042: (1992). 6. Mauzy, C.A., et al., BBRC 183: 8 (1992). 7. Bergsma, D.J., et al., BBRC 183: 989 (1992). 8. Konishi, H., et al., BBRC 199: 467 (1992). 9. Iwai, N., et al., FEBS Lett. 298: 257 (1992). 10. Sandberg, K., et al., BBRC 183: 1090 (1992). 11. Elton, T.S., et al., BBRC 184: 1067 (1992). 12. Hahn, A.W., et al., BBRC 192: 1260 (1993). \nCitations: \nStewart JA; 2006; J Mol Cell Cardiol, 41, 97-107; WB; Hoffman A; 2004; J. Recept Signal Transduction, 23 1; WB; IHC Javeshghani D; 2003; Hypertension, 42, 761-767; WB; Nadal JA; 2002; Am. J. Physiol 282, H739-H748; WB,; Javeshghani D; 2003; Hypertension, 42, 761-767; WB; Zeng C; 2002; Hypertension, 41, 724-729; WB; Jin X-Q; 2002; Hypertension. 39:1021-1027; WB; Nithipatikom K; 2004; AJPEM, 287: 820 - 827; WB,; IF \nSu J-Z; 2002; Hypertension : 40, 853-858; WB; Javeshghani D; 2003; Hypertension, 42, 761-767; WB; Hussain T; 1998; Hypertension 32: 1054-1059; WB,; Rodriguez-Iturbe B; 2001; Kidney Intl. 59, 6, 2222; ; IHC, Lee Jongun; 2002; Pharmacological Res. 46, 5, 383-387; WB; Hakam AC; 2006; AJEP, 290: F503 - F508.; WB;

Additional Info

Recognizes rat Angiotensin II type 1 Receptor. Sequence is the same in all AT II receptor isoforms (Type 1a, 1b and 1c). No sequence homology with other G-protein coupled receptors. Species sequence homology: mouse, human, porcine, sheep, canine, chimpanzee and rabbit: 100%.