Angiotensin II Type 2 Receptor (AT2)

Angiotensin II mediates its action by interacting with membrane receptor, Angiotensin II type 1 receptors. The signal is transmitted via G-proteins that activate a phosphatidylinositol- calcium second messenger system. ATII receptors are integral membrane proteins of approx. 359-363aa and predicted to contain at least 7 transmembrane domains. The N and C-terminus are predicted to extracellular and cytoplasmic, respectively. Three isoforms of ATII receptors have been cloned. In rat, type 1a (359aa) is expressed in the liver kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscles. Rat Type 1b (also known as AT3; 359aa) is found in liver, kidney, and aorta. Type 1c (177aa) has been detected in brain, kidney, liver, spleen and lung.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL). Detects ~45kD band.\nELISA: 1:10,000-1:100,000 using 50-100ng of A2295-07U control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2295-07S

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Martin, M.M., et al., BBRC 209: 554-562 (1995). 2. Chassagne, C., et al., Genomics 25: 601-603 (1995). 3. Koike, G., et al., BBRC 203: 1842-50 (1994). 4. Tsuzuki, S., et al., BBRC 200: 1449-54 (1994). 5. Martin, M.M., et al., BBRC 205: 645-651 (1994). 6. Lazard, D., et al., Receptor Channels 2: 271-280 (1994).

Additional Info

Recognizes human AT2 receptor. No sequence homology with other G-protein coupled receptors. Species Sequence Homology: Rabbit: 100%; rat: 88%; mouse: 83%.