Angiotensinogen (AGT, ANHU, SERPINA8)

Angiotensinogen is a precursor of angiotensin-1 and a member of serpin family, expressed by the liver and secreted in plasma. Angiotensinogen contains Angiotensin 1, 2, and 3. In response to lowered blood pressure, remin cleaves Angiotensinogen and produce angiotensin-1 (1). Angiotensin-converting enzyme (ACE) further removes a dipeptide to produce the physiologically active peptide angiotensin-2. Angiotensin-2 is involved in regulating mineral balance and volume of body fluids, while Angiotensin-3 stimulates aldosterone release (2). In human, Angiotensinogen is 118 amino acids long, but its size varies between species. Defects in Angiotensinogen have been linked to renal tubular dygenesis (RTD) and susceptibility to essential hypertension (3).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nWestern Blot: 1:200,000-1:500,000\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A2295-10F

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Tainmoto K et al. JBC, 269(50):31334-7, 1994 2. Kilian P et al. Endocrinology 149 (6):2923-2933, 2008 3. The UniProt Consortium, The Universal Protein Resource (UniProt) Nucleic Acids Res. 36:D190-D195 (2008)

Additional Info

Recognizes human Angiotensinogen.