Angiotensinogen is a precursor of angiotensin-1 and a member of serpin family, expressed by the liver and secreted in plasma. Angiotensinogen contains Angiotensin 1, 2, and 3. In response to lowered blood pressure, remin cleaves Angiotensinogen and produce angiotensin-1 (1). Angiotensin-converting enzyme (ACE) further removes a dipeptide to produce the physiologically active peptide angiotensin-2. Angiotensin-2 is involved in regulating mineral balance and volume of body fluids, while Angiotensin-3 stimulates aldosterone release (2). In human, Angiotensinogen is 118 amino acids long, but its size varies between species. Defects in Angiotensinogen have been linked to renal tubular dygenesis (RTD) and susceptibility to essential hypertension (3).
Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:200,000-1:500,000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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