ANGPTL7, aa1-346, Recombinant Human (Angiopoietin-related Protein 7, Angiopoietin-like Protein 7, Angiopoietin-like Factor, Cornea-derived Transcript 6 Protein, CDT6, UNQ313/PRO35)

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1-3). ANGPTL7 shares 89aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45-50kD monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up-regulated in trabecular meshwork cells by glucocorticoids and TGF-B and in cartilage by TNF-a (5-7). Overexpression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When overexpressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells (9).\n\nSource: \nRecombinant corresponding to aa1-346 from human Angiopoietin-like Protein 7/ANGPTL7, fused with 10-His tag at C-terminal, expressed in CHO cells.\n\nMolecular Weight: \n~38.6kD\n\nEndotoxin Level: \n(same/less than)1EU/1ug (LAL)\n\nBiological Activity:\nMeasured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of recombinant mouse SCF, recombinant mouse Thrombopoietin, and recombinant mouse Flt-3 Ligand. The ED50 for this effect is typically 50-200ng/mL in the presence of a cross-linking antibody, Mouse polyHistidine monoclonal antibody.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2293-52K

Size

25ug

Form

Supplied as a lyophilized powder in PBS, sodium chloride, BSA. Reconstitute with PBS to 100ug/ml.

Purity

~95% (SDS-PAGE)

References

1. Oike, Y. et al. (2009) Circ. J. 73:2192. 2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70. 3. Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782. 4. Peek, R. et al. (2002) J. Biol. Chem. 277:686. 5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438. 6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58. 7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489. 8. Comes, N. et al. (2010) Genes Cells 16:243. 9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.