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Anillin (ANLN, Actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Scraps)

Anillin (ANLN, Actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Scraps)

Cat no: A2295-12X3

Supplier: United States Biological
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Anillin is an actin-binding protein that can bind septins and is a component of the cytokinetic ring. Anillin is expressed ubiquitously but with variable levels of expression, being highest in the central nervous system. Anillin is overexpressed in diverse common human tumors, but not simply as a consequence of being a proliferation marker. Anillin may have potential as a novel biomarker. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:4000 Western Blot: 1ug/ml. We cannot find an explanation in the literature for the bands we observe given the calculated size of 124kD according to NP_061155.2. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2295-12X3
Reactivities: Human, Mouse
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Oegema K, Savoian MS, Mitchison TJ, Field CM. Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis. J Cell Biol. 2000 Aug 7;150(3):539-52.
Additional info: Recognizes human ANILLIN. Species Crossreactivity: Mouse.

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