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Anillin (ANLN, Actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Scraps)

Anillin (ANLN, Actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Scraps)

Cat no: A2295-12X4

Supplier: United States Biological
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Anillin is an actin-binding protein that can bind septins and is a component of the cytokinetic ring. Anillin is expressed ubiquitously but with variable levels of expression, being highest in the central nervous system. Anillin is overexpressed in diverse common human tumors, but not simply as a consequence of being a proliferation marker. Anillin may have potential as a novel biomarker. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2295-12X4
Reactivities: Human, Mouse, Rat, Canine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Hall PA, Todd CB, Hyland PL, McDade SS, Grabsch H, Dattani M, Hillan KJ, Russell SE. The septin-binding protein anillin is overexpressed in diverse human tumors. Clin Cancer Res. 2005 Oct 1;11(19 Pt 1):6780-6.
Additional info: Recognizes ANILLIN at ~100kD in Human and Mouse Brain lysates (calculated MW of 124kD according to NP_061155.2). Species Crossreactivity: Canine, mouse and rat.

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