Anion Exchanger 3 (AE3, Anion exchange protein 3, CAE3/BAE3, Cardiac/brain band 3-like protein, Neuronal band 3-like protein, SLC2C, Solute carrier family 4 member 3)

Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself. Human AE3 gene (chromosome 2q36) encodes a protein of 1233aa (mouse/rat 1227aa). AE3 is primarily expressed in excitable tissue such cardiac muscle, retina, and brain.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000 (ECL)\nELISA: 1:10,000-1:50,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2295-14F

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kudrycki KE et al (1990) J Biol. Chem. 265, 462. 2. Kopito RR et al (1989) Cell 59, 927. 3. Morgan CW et al (1993) J Cell Sci. 106, 1275. 4. Yannoukakos D et al (1994) Cir. Res. 75, 603. 5. Kopito RR et al (1990) Intl. Rev. Cytol. 123, 177.

Additional Info

Recognizes rat AE3. No significant sequence homology is detected with other AE or other proteins. Does not recognize cardiac AE3.