Annexin A2 (ANXA2, annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polype

Alternate Name: ANXA2, annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polypeptide), calpactin I heavy polypeptide, chromobindin 8, lipocortin II, placental anticoagulant protein IV\n\nDomain Information: Annexin\n\nGene Ontology Terms: phospholipase inhibitor activity; calcium-dependent phospholipid binding; calcium ion binding; skeletal development; soluble fraction; plasma membrane; anticoagulant activity\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 35kD band observed in lysates of Human cell lines A549 and HeLa (calculated MW of 40.4kD according to NP_001002858.1 and 38.6kD according to NP_004030.1 and NP_001002857.1). Recommended concentration: 0.1-0.3ug/ml.\n\nStaining (0.1ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See A2295-15C-P. Antibody

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SPECIFICATIONS

Catalog Number

A2295-15C

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Prc

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Takahashi S, Reddy SV, Chirgwin JM, Devlin R, Haipek C, Anderson J, Roodman GD., Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption., J Biol Chem 1994 Nov 18;269(46):28696-701

Additional Info

This antibody is expected to recognize both reproted isoforms. NP_004030.1 and NP_001002857.1 are variants of isoform 2. Species Crossreactivity: human, mouse, rat, bovine porcine.