Annexin A5 (Anchorin CII, Annexin V, Annexin-5, Calphobindin I)

Annexin V also know as endonnexin II?ENX2?,or placental protein 4 (PP4). Endonexin II is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.\n\nUniProt Number:\nP08758\n\nGene ID:\nANXA5\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144167

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kaplan, R.; Jaye, M.; Burgess, W. H.; Schlaepfer, D. D.; Haigler, H. T. : Cloning and expression of cDNA for human endonexin II, a Ca(2+) and phospholipid binding protein. J. Biol. Chem. 263\n2. Rodriguez-Garcia, M. I.; Kozak, C. A.; Morgan, R. O.; Fernandez, M. P. : Mouse annexin V chromosomal localization, cDNA sequence conservation, and molecular evolution. Genomics 31: 151-157, 1996. \n3. Grundmann, U.; Abel, K.-J.; Bohn, H.; Lobermann, H.; Lottspeich, F.; Kupper, H. : Characterization of cDNA encoding human placental anticoagulant protein (PP4): homology with the lipocortin family. Proc. Nat. Acad. Sci. 85: 3708-3712, 1988.

Additional Info

Recognizes human, mouse and rat Annexin A5. No crossreactivity with other proteins.