Annexin A8 (Annexin VIII, Annexin-8, Vascular anticoagulant-beta)

Human Annexin A8 was originally identified from a 2-kb cDNA transcript containing an open reading frame that encoded a 327-amino acid protein termed vascular anticoagulant beta. It is a minor annexin in human placenta and shows restricted expression in lung endothelia, skin, liver, and kidney. The gene is selectively overexpressed in acute myelocytic leukemia. The Annexin A8 gene is mapped to chromosome 10q11.2 by fluorescence in situ hybridization. Affected human promyelocytes contain a nonrandom chromosomal translocation breakpoint involving chromosomes 15 and 17 and creating 2 hybrid mRNA fusion products and chimeric proteins. These involve the retinoic acid receptor-alpha geneon chromosome 17q12 and the promyelocytic leukemia gen on chromosome 15q22. Strong overexpression of Annexin A8 in this disorder could be repressed by retinoic acid-induced expression of the RARA gene product.\n\nUniProt Number:\nP13928\n\nGene ID:\nANXA8\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144169

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Chambers, J. A., Gardner, E., Hauptmann, R., Ponder, B. A., Mulligan, L. M.TaqI polymorphisms at the annexin VIII locus (ANX8).Hum. Molec. Genet. 1: 550 only, 1992.\n2. Chang, K.-S., Wang, G., Freireich, E. J., Daly, M., Naylor, S. L., Trujillo, J. M., Stass, S. A.Specific expression of the annexin VIII gene in acute promyelocytic leukemia.Blood 79: 1802-1810, 1992.\n3. Sarkar, A., Yang, P., Fan, Y.-H., Mu, Z. M., Hauptmann, R., Adolf, G. R., Stass, S. A., Chang, K.-S.Regulation of the expression of annexin VIII in acute promyelocytic leukemia.Blood 84: 279-286, 1994.\n

Additional Info

Recognizes human and rat Annexin A8. No crossreactivity with other proteins.