Annexin I (Annexin A1, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 Inhibitory Protein, p35, ANXA1, ANX1, LPC1)

The Annexins are a structurally related family of calcium-binding proteins that associate reversibly with membranes, and bind to phospholipid bilayers in the presence of micromolar free calcium concentrations. This binding is speci?c for calcium and for acidic phospholipids. Each of the annexin proteins consist of an N-terminal domain of variable length followed by four or eight copies of a conserved segment of sixty one residues. The repeat (sometimes known as an 'endonexin fold') consists of ?ve alpha-helices that are wound into a right-handed superhelix. Annexins have been demonstrated to be involved in cytoskeletal interactions, phospholipase inhibition, intracellular signaling, anticoagulation, and membrane fusion. Although the annexins are primarily intracellular proteins and lack hydrophobic signal sequences, some family members (e.g. Annexin I and V) have been found to be secreted. Annexin I (calpactin II, chromobindin 9, GIF, Lipocortin, p35) is a cytoskeletal calcium-dependent phospholipid-binding protein which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and is may be involved in secretion. Although this protein has received considerable attention due to its potential involvement in a variety of pathways, research progress on the localization of Annexin I during development and in pathophysiological conditions has been hampered due to con?icting reports on the immunolocalization of the protein. These contradictory data are believed to result in large part from unusual properties of the protein and its apparent sensitivity to different ?xation conditions.\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:1-2ug/ml \nImmunohistochemistry (paraffin):1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2295-15A3

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Wallner, B. P.; Mattaliano, R. J.; Hession, C.; Cate, R. L.; Tizard, R.; Sinclair, L. K.; Foeller, C.; Chow,E. P.; Browning, J. L.; Ramachandran, K. L.; Pepinsky, R. B. : Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320: 77-81,1986. 2. Kovacic RT, Tizard R, Cate RL, Frey AZ, Wallner BP. Correlation of gene and protein structure of rat and human lipocortin I. Biochemistry. 1991 Sep 17;30(37):9015-21. 3. Walther, A.; Riehemann, K.; Gerke, V. A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000.

Additional Info

Recognizes human Annexin I.