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Anterior Gradient 2, ID (Anterior Gradient Protein 2 Homolog, AG-2, hAG-2, HPC8, Secreted Cement Gland Protein XAG-2 Homolog, AGR2, AG2, UNQ515/PRO1030)

Cat no: A2296-82F

Anterior Gradient 2, ID (Anterior Gradient Protein 2 Homolog, AG-2, hAG-2, HPC8, Secreted Cement Gland Protein XAG-2 Homolog, AGR2, AG2, UNQ515/PRO1030)

AG-2 (Anterior gradient protein 2; also AGR2, GOB4 and HPC8) is an 18-21kD member of the PDI family of enzymes. It is expressed in secretory cell types, such as prostate epithelium, small intestine goblet, Paneth and enteroendocrine cells, and multiple carcinoma cell types. Although AG-2 possesses a signal sequence, it appears to be rarely secreted, instead being found in the ER due the presence of a KTEL ER retention signal at its C-terminus. AG-2 forms transient disulfide linkages with molecules destined for secretion, possibly aiding protein folding. Mature human AG-2 is 155aa in length (aa21-175). Cys81 is presumed to participate in intermolecular bond formation. There is one potential alternative start site 20aa upstream of the standard start site.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:128,000\nWestern Blot: 1:128,000\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2296-82F

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human AGR2. Species sequence homology: Canine and mouse.

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